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Western Blot analysis of COLO205 cells using KMT1B Polyclonal Antibody
Western Blot analysis of COLO205 cells using KMT1B Polyclonal Antibody
Western Blot analysis of COLO205 cells using KMT1B Polyclonal Antibody

SUV39H2 Antibody

CSB-PA003106
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSUV39H2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SUV39H2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79723
  • Target name
    SUV39H2
  • Target description
    SUV39H2 histone lysine methyltransferase
  • Target synonyms
    H3-K9-HMTase 2; histone H3-K9 methyltransferase 2; histone methyltransferase SUV39H2; histone-lysine N-methyltransferase SUV39H2; KMT1B; lysine N-methyltransferase 1B; su(var)3-9 homolog 2; suppressor of variegation 3-9 homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H5I1
  • Protein Name
    Histone-lysine N-methyltransferase SUV39H2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KMT1B/SUV39H2 using anti-KMT1B/SUV39H2 antibody (A05361-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT1B/SUV39H2 antigen affinity purified polyclonal antibody (Catalog # A05361-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT1B/SUV39H2 at approximately 47 kDa. The expected band size for KMT1B/SUV39H2 is at 47 kDa.
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