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SUV39H2 Antibody

LS-C832508
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSUV39H2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SUV39H2 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.02 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79723
  • Target name
    SUV39H2
  • Target description
    SUV39H2 histone lysine methyltransferase
  • Target synonyms
    H3-K9-HMTase 2; histone H3-K9 methyltransferase 2; histone methyltransferase SUV39H2; histone-lysine N-methyltransferase SUV39H2; KMT1B; lysine N-methyltransferase 1B; su(var)3-9 homolog 2; suppressor of variegation 3-9 homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KMT1B/SUV39H2 using anti-KMT1B/SUV39H2 antibody (A05361-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT1B/SUV39H2 antigen affinity purified polyclonal antibody (Catalog # A05361-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT1B/SUV39H2 at approximately 47 kDa. The expected band size for KMT1B/SUV39H2 is at 47 kDa.
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