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KMT6/EZH2 Antibody (YA320, Rabbit)

HY-P80507
MedChem Express
TargetEZH2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    KMT6/EZH2 Antibody (YA320, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2146
  • Target name
    EZH2
  • Target description
    enhancer of zeste 2 polycomb repressive complex 2 subunit
  • Target synonyms
    enhancer of zeste homolog 2; ENX1; ENX-1; EZH2b; histone-lysine N-methyltransferase EZH2; KMT6; KMT6A; lysine N-methyltransferase 6; WVS; WVS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    KMT6/EZH2 Antibody (YA320) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KMT6/EZH2.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Western blot All lanes: Histone-lysine N-methyltransferase EZH2 antibody at 12ug/ml Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 86, 87, 82, 85, 80 kDa Observed band size: 86 kDa
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Figure 1. Western blot analysis of KMT6/EZH2 using anti-KMT6/EZH2 antibody (A00050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT6/EZH2 antigen affinity purified polyclonal antibody (Catalog # A00050-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT6/EZH2 at approximately 98 kDa. The expected band size for KMT6/EZH2 is at 85 kDa.
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GeneTex
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