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[KO Validated] CSPG4 Antibody

CAB3592
Assay Genie
TargetCSPG4
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] CSPG4 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1464
  • Target name
    CSPG4
  • Target description
    chondroitin sulfate proteoglycan 4
  • Target synonyms
    chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan NG2; CSPG4A; HMW-MAA; MCSP; MCSPG; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; MEL-CSPG; MSK16; NG2
  • Host
    Rabbit
  • Protein IDQ6UVK1
  • Protein Name
    Chondroitin sulfate proteoglycan 4
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NG2/CSPG4 using anti-NG2/CSPG4 antibody (A03394-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NG2/CSPG4 antigen affinity purified polyclonal antibody (Catalog # A03394-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NG2/CSPG4 at approximately 310 kDa. The expected band size for NG2/CSPG4 is at 251 kDa.
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