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ProductsBack/[KO Validated] HPRT1 Rabbit mAb

[KO Validated] HPRT1 Rabbit mAb

A8783
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHPRT1
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Overview

  • Supplier
    ABclonal
  • Product Name
    [KO Validated] HPRT1 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID3251
  • Target name
    HPRT1
  • Target description
    hypoxanthine phosphoribosyltransferase 1
  • Target synonyms
    HGPRT, HPRT, hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, hypoxanthine guanine phosphoribosyl transferase, hypoxanthine-guanine phosphoribosyltransferase 1, testicular tissue protein Li 89
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00492
  • Protein Name
    Hypoxanthine-guanine phosphoribosyltransferase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HPRT1 using anti-HPRT1 antibody (A00668-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified polyclonal antibody (Catalog # A00668-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 25 kDa. The expected band size for HPRT1 is at 25-28 kDa.
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