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ProductsBack/[KO Validated] Lsh/HELLS Rabbit pAb

[KO Validated] Lsh/HELLS Rabbit pAb

A5831
ABclonal
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHELLS
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Overview

  • Supplier
    ABclonal
  • Product Name
    [KO Validated] Lsh/HELLS Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    WB,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3070
  • Target name
    HELLS
  • Target description
    helicase, lymphoid specific
  • Target synonyms
    ICF4, LSH, Nbla10143, PASG, SALNR, SMARCA6, lymphoid-specific helicase, SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6, Senescence Associated Long Non-coding RNA, proliferation-associated SNF2-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NRZ9
  • Protein Name
    Lymphoid-specific helicase
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HELLS using anti-HELLS antibody (A04702-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HELLS antigen affinity purified polyclonal antibody (Catalog # A04702-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HELLS at approximately 97 kDa. The expected band size for HELLS is at 97 kDa.
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