More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Smad2 Antibody -Knockout Validated

Anti-Smad2 Antibody -Knockout Validated

CAB7699
Assay Genie
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSMAD2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-Smad2 Antibody -Knockout Validated
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID4087
  • Target name
    SMAD2
  • Target description
    SMAD family member 2
  • Target synonyms
    CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, mothers against decapentaplegic homolog 2, MAD homolog 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
  • Host
    Rabbit
  • Protein IDQ15796
  • Protein Name
    Mothers against decapentaplegic homolog 2
  • Scientific Description
    [KO Validated] Smad2 Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, IHC-P, IF/ICC, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Anti-Smad2 Antibody
Product group Antibodies
Antibodies.com
Anti-Smad2 AntibodyA95209
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-SMAD2 [RAB-S220]Ab01874-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityHuman
TargetSMAD2
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-SMAD2 (human), mAb (rec.) (PAS4-G7)AG-27B-6329
ApplicationsELISA, ImmunoCytoChemistry, Other Application
ReactivityHuman
TargetSMAD2
  • SizePrice
Immunohistochemistry analysis in human fallopian tube and pancreas tissues using AMAb91520 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-SMAD2 AntibodyAMAB91520
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSMAD2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SMAD2 AntibodyLS-C761093
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetSMAD2
  • SizePrice
Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: rat liver tissue lysates Lane 3: mouse testicular tissue lysates Lane 4: mouse heart tissue lysates Lane 5: mouse lung tissue lysates Lane 6: mouse lung tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.
Product group Antibodies
Boster Bio
Anti-SMAD2 Antibody Picoband(r)A00090-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2(Ser465 + Ser467) Polyclonal Antibody, Unconjugated (bs-3419R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
References
Bioss
Phospho-Smad2 (Ser465 + Ser467) Polyclonal AntibodyBS-3419R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat
TargetSMAD2
  • SizePrice
Western Blot analysis of HepG2 cells using Smad2 Polyclonal Antibody
Product group Antibodies
Cusabio
SMAD2 AntibodyCSB-PA004106
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice
Product group Antibodies
Biomatik
SMAD2 Polyclonal AntibodyCAC14557
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetSMAD2
  • SizePrice
SMAD2 antibody detects SMAD2 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. SMAD2 antibody (GTX111075) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
SMAD2 antibodyGTX111075
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice