More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/[KO Validated] UBE2O Antibody

[KO Validated] UBE2O Antibody

CAB10036
Assay Genie
ReactivityHuman
Product group Antibodies
TargetUBE2O
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] UBE2O Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID63893
  • Target name
    UBE2O
  • Target description
    ubiquitin conjugating enzyme E2 O
  • Target synonyms
    E2-230K, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, ubiquitin carrier protein O, ubiquitin conjugating enzyme E2O, ubiquitin-conjugating enzyme E2 of 230 kDa, ubiquitin-conjugating enzyme E2-230K, ubiquitin-protein ligase O
  • Host
    Rabbit
  • Protein IDQ9C0C9
  • Protein Name
    (E3-independent) E2 ubiquitin-conjugating enzyme
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-CLSPN Antibody144-64837
ApplicationsWestern Blot
ReactivityHuman
TargetUBE2O
  • SizePrice
UBE2O antibody [C1C2], Internal detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. UBE2O antibody [C1C2], Internal (GTX119315) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
References
GeneTex
UBE2O antibody [C1C2], InternalGTX119315
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetUBE2O
  • SizePrice
Product group Antibodies
Biomatik
UBE2O Polyclonal AntibodyCAC14409
ApplicationsWestern Blot, ELISA
TargetUBE2O
  • SizePrice
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (UBE2O) polyclonal Antibody, Unconjugated (bs-55219) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Product group Antibodies
Bioss
UBE2O Polyclonal AntibodyBS-55219R
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetUBE2O
  • SizePrice
Anti-UBE2O Antibody
Product group Antibodies
Antibodies.com
Anti-UBE2O AntibodyA11220
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Abbexa
E2-230K AntibodyABX430782
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-E2-230K / UBE2OEB05761
ApplicationsELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetUBE2O
  • SizePrice
Figure 1. Western blot analysis of UBE2O using anti-UBE2O antibody (A09217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2O antigen affinity purified polyclonal antibody (Catalog # A09217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2O at approximately 200 kDa. The expected band size for UBE2O is at 141 kDa.
Product group Antibodies
Boster Bio
Anti-UBE2O Antibody Picoband(r)A09217-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Rat
TargetUBE2O
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
UBE2O AntibodyLS-C668583
ApplicationsWestern Blot
ReactivityHuman
TargetUBE2O
  • SizePrice
Western blot All lanes: UBE2O antibody at 0.8microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 142 kDa Observed band size: 142 kDa
Product group Antibodies
Cusabio
UBE2O AntibodyCSB-PA866339LA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetUBE2O
  • SizePrice