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KTN1 Rabbit pAb

A5879
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKTN1
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Overview

  • Supplier
    ABclonal
  • Product Name
    KTN1 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3895
  • Target name
    KTN1
  • Target description
    kinectin 1
  • Target synonyms
    CG1, KNT, MU-RMS-40.19, kinectin, CG-1 antigen, kinesin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UP2
  • Protein Name
    Kinectin
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Kinectin 1/KTN1 using anti-Kinectin 1/KTN1 antibody (A05071-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kinectin 1/KTN1 antigen affinity purified polyclonal antibody (Catalog # A05071-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kinectin 1/KTN1 at approximately 160 kDa. The expected band size for Kinectin 1/KTN1 is at 160 kDa.
Product group Antibodies
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Anti-Kinectin 1/KTN1 Antibody Picoband(r)A05071-1-CARRIER-FREE
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ReactivityHuman, Monkey, Mouse, Rat
TargetKTN1
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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: KTN1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 150, 151, 153 kDa Observed band size: 157 kDa
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Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Kinectin 1 Polyclonal Antibody, Unconjugated (bs-17064R) at 1:400 overnight at 4°C, DAB staining.
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TargetKTN1
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Immunohistochemistry analysis in human testis and bone marrow tissues using Anti-KTN1 antibody. Corresponding KTN1 RNA-seq data are presented for the same tissues.
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