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Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n
Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n
Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n

Lactoferrin Polyclonal Antibody

BS-5810R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLTF
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Overview

  • Supplier
    Bioss
  • Product Name
    Lactoferrin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4057
  • Target name
    LTF
  • Target description
    lactotransferrin
  • Target synonyms
    GIG12, HEL110, HLF2, LF, lactotransferrin, epididymis luminal protein 110, growth-inhibiting protein 12, kaliocin-1, lactoferricin, lactoferroxin, neutrophil lactoferrin, talalactoferrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhao X, Ting SM, Liu CH, et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. Nat Commun. 2017,8(1):602. doi: 10.1038/s41467-017-00770-7
    Read this paper

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Figure 1. Western blot analysis of Lactoferrin using anti-Lactoferrin antibody (A00633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lactoferrin antigen affinity purified polyclonal antibody (Catalog # A00633-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lactoferrin at approximately 85 kDa. The expected band size for Lactoferrin is at 78 kDa.
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