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Western Blot analysis of K562 cells using Laminin gamma-2 Polyclonal Antibody.
Western Blot analysis of K562 cells using Laminin gamma-2 Polyclonal Antibody.
Western Blot analysis of K562 cells using Laminin gamma-2 Polyclonal Antibody.

LAMC2 Antibody

CSB-PA005613
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLAMC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LAMC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3918
  • Target name
    LAMC2
  • Target description
    laminin subunit gamma 2
  • Target synonyms
    B2T; BM600; BM600-100kDa; cell-scattering factor 140 kDa subunit; CSF; CSF 140 kDa subunit; EBR2; EBR2A; epiligrin subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; LAMB2T; laminin B2t chain; laminin subunit gamma-2; laminin, gamma 2; LAMNB2; large adhesive scatter factor 140 kDa subunit; nicein subunit gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13753
  • Protein Name
    Laminin subunit gamma-2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LAMC2 using anti-LAMC2 antibody (A03214). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMC2 antigen affinity purified polyclonal antibody (Catalog # A03214) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMC2 at approximately 131 kDa. The expected band size for LAMC2 is at 131 kDa.
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