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ProductsBack/LAMC2 / Laminin Gamma 2 Antibody (C-Terminus)

LAMC2 / Laminin Gamma 2 Antibody (C-Terminus)

LS-C368946
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLAMC2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LAMC2 / Laminin Gamma 2 Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3918
  • Target name
    LAMC2
  • Target description
    laminin subunit gamma 2
  • Target synonyms
    B2T, BM600, CSF, EBR2, EBR2A, JEB3A, JEB3B, LAMB2T, LAMNB2, laminin subunit gamma-2, BM600-100kDa, CSF 140 kDa subunit, cell-scattering factor 140 kDa subunit, epiligrin subunit gamma, kalinin subunit gamma, ladsin 140 kDa subunit, laminin B2t chain, laminin, gamma 2, large adhesive scatter factor 140 kDa subunit, nicein subunit gamma
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LAMC2 using anti-LAMC2 antibody (A03214). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMC2 antigen affinity purified polyclonal antibody (Catalog # A03214) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMC2 at approximately 131 kDa. The expected band size for LAMC2 is at 131 kDa.
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