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LAMP2 antibody detects LAMP2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
LAMP2 antibody detects LAMP2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
LAMP2 antibody detects LAMP2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

LAMP2 antibody

GTX103214
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLAMP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    LAMP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.42 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3920
  • Target name
    LAMP2
  • Target description
    lysosomal associated membrane protein 2
  • Target synonyms
    CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110, lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13473
  • Protein Name
    Lysosome-associated membrane glycoprotein 2
  • Scientific Description
    The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (Catalog # A01573-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP2 at approximately 100-110 kDa. The expected band size for LAMP2 is at 45 kDa.
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