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LAR Polyclonal Antibody

BS-11530R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTPRF
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Overview

  • Supplier
    Bioss
  • Product Name
    LAR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5792
  • Target name
    PTPRF
  • Target description
    protein tyrosine phosphatase receptor type F
  • Target synonyms
    BNAH2, LAR, receptor-type tyrosine-protein phosphatase F, LCA-homolog, leukocyte antigen-related (LAR) PTP receptor, leukocyte antigen-related tyrosine phosphatase, leukocyte common antigen related, protein tyrosine phosphatase, receptor type, F polypeptide, receptor-linked protein-tyrosine phosphatase LAR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10586
  • Protein Name
    Receptor-type tyrosine-protein phosphatase F
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of LAR using anti-LAR antibody (PB9786). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: A431 Whole Cell Lysate, Lane 3: A549 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAR antigen affinity purified polyclonal antibody (Catalog # PB9786) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAR at approximately 240 kDa. The expected band size for LAR is at 240 kDa.
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