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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA019053LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA019053LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA019053LA01HU at dilution of 1:100

PTPRF Antibody

CSB-PA019053LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTPRF
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTPRF Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5792
  • Target name
    PTPRF
  • Target description
    protein tyrosine phosphatase receptor type F
  • Target synonyms
    BNAH2, LAR, receptor-type tyrosine-protein phosphatase F, LCA-homolog, leukocyte antigen-related (LAR) PTP receptor, leukocyte antigen-related tyrosine phosphatase, leukocyte common antigen related, protein tyrosine phosphatase, receptor type, F polypeptide, receptor-linked protein-tyrosine phosphatase LAR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10586
  • Protein Name
    Receptor-type tyrosine-protein phosphatase F
  • Scientific Description
    Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LAR using anti-LAR antibody (PB9786). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: A431 Whole Cell Lysate, Lane 3: A549 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAR antigen affinity purified polyclonal antibody (Catalog # PB9786) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAR at approximately 240 kDa. The expected band size for LAR is at 240 kDa.
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