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LC3A Antibody (YA4496, Mouse)

HY-P84799
MedChem Express
TargetMAP1LC3A
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    LC3A Antibody (YA4496, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID84557
  • Target name
    MAP1LC3A
  • Target description
    microtubule associated protein 1 light chain 3 alpha
  • Target synonyms
    ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, microtubule-associated protein 1 light chain 3 alpha, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, autophagy-related ubiquitin-like modifier LC3 A, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    LC3A Antibody (YA4496) is a mouse-derived and non-conjugated IgG1 antibody, targeting to MAP1LC3A. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A antigen affinity purified polyclonal antibody (Catalog # A01543-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP1LC3A at approximately 18KD. The expected band size for MAP1LC3A is at 14KD.
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