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ProductsBack/LCN2 / Lipocalin 2 / NGAL Antibody (clone 5H3-H12-E12-E10-E3)

LCN2 / Lipocalin 2 / NGAL Antibody (clone 5H3-H12-E12-E10-E3)

LS-C744441
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLCN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LCN2 / Lipocalin 2 / NGAL Antibody (clone 5H3-H12-E12-E10-E3)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:320000), WB (1:1000 - 1:5000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H3-H12-E12-E10-E3
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3934
  • Target name
    LCN2
  • Target description
    lipocalin 2
  • Target synonyms
    24p3, MSFI, NGAL, p25, neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, migration-stimulating factor inhibitor, oncogene 24p3, siderocalin LCN2
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: A549 Whole Cell Lysate at 40ug, Lane 2: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9610) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22 kDa. The expected band size for Lipocalin 2 is at 22 kDa.
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