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ProductsBack/Lipocalin 2/NGAL Polyclonal Antibody
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti NGAL/Lipocalin 2 Polyclonal Antibody, Unconjugated (bs-1373R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti NGAL/Lipocalin 2 Polyclonal Antibody, Unconjugated (bs-1373R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti NGAL/Lipocalin 2 Polyclonal Antibody, Unconjugated (bs-1373R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lipocalin 2/NGAL Polyclonal Antibody

BS-1373R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLCN2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Lipocalin 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3934
  • Target name
    LCN2
  • Target description
    lipocalin 2
  • Target synonyms
    24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; oncogene 24p3; p25; siderocalin LCN2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP80188
  • Protein Name
    Neutrophil gelatinase-associated lipocalin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: A549 Whole Cell Lysate at 40ug, Lane 2: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9610) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22 kDa. The expected band size for Lipocalin 2 is at 22 kDa.
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