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ProductsBack/LDLR / LDL Receptor Antibody (aa1-790, clone 004, Preservative Free)

LDLR / LDL Receptor Antibody (aa1-790, clone 004, Preservative Free)

LS-C819365
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetLDLR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LDLR / LDL Receptor Antibody (aa1-790, clone 004, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:20 - 1:100), IF (1:20 - 1:100) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    004
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3949
  • Target name
    LDLR
  • Target description
    low density lipoprotein receptor
  • Target synonyms
    FH, FHC, FHCL1, LDLCQ2, low-density lipoprotein receptor, LDL receptor, low-density lipoprotein receptor class A domain-containing protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LDLR using anti-LDLR antibody (A00076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDLR antigen affinity purified polyclonal antibody (Catalog # A00076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDLR at approximately 130KD. The expected band size for LDLR is at 95KD.
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