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Mouse lymph node lysates probed with Rabbit Anti-LEF-1 Polyclonal Antibody, Unconjugated (bs-1843R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse lymph node lysates probed with Rabbit Anti-LEF-1 Polyclonal Antibody, Unconjugated (bs-1843R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse lymph node lysates probed with Rabbit Anti-LEF-1 Polyclonal Antibody, Unconjugated (bs-1843R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

LEF-1 Polyclonal Antibody

BS-1843R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLEF1
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Overview

  • Supplier
    Bioss
  • Product Name
    LEF-1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID51176
  • Target name
    LEF1
  • Target description
    lymphoid enhancer binding factor 1
  • Target synonyms
    ECTD1, ECTD17, LEF-1, TCF10, TCF1ALPHA, TCF7L3, lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJU2
  • Protein Name
    Lymphoid enhancer-binding factor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LEF1 using anti-LEF1 antibody (A00605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEF1 antigen affinity purified polyclonal antibody (Catalog # A00605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LEF1 at approximately 58 kDa. The expected band size for LEF1 is at 58 kDa.
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