More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/LEF1 antibody
LEF1 antibody detects LEF1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody (GTX129186) diluted by 1:1000.
LEF1 antibody detects LEF1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody (GTX129186) diluted by 1:1000.
LEF1 antibody detects LEF1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody (GTX129186) diluted by 1:1000.

LEF1 antibody

GTX129186
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetLEF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    LEF1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51176
  • Target name
    LEF1
  • Target description
    lymphoid enhancer binding factor 1
  • Target synonyms
    ECTD1, ECTD17, LEF-1, TCF10, TCF1ALPHA, TCF7L3, lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJU2
  • Protein Name
    Lymphoid enhancer-binding factor 1
  • Scientific Description
    LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) (Waterman et al., 1991 [PubMed 2010090]; van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM]
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
LEF1 AntibodyCAB0909
ReactivityHuman
TargetLEF1
  • SizePrice
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: LEF1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 36, 32, 24, 42, 43, 35 kDa Observed band size: 45 kDa
Product group Antibodies
Cusabio
LEF1 AntibodyCSB-PA012856LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetLEF1
  • SizePrice
Figure 1. Western blot analysis of LEF1 using anti-LEF1 antibody (A00605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEF1 antigen affinity purified polyclonal antibody (Catalog # A00605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LEF1 at approximately 58 kDa. The expected band size for LEF1 is at 58 kDa.
Product group Antibodies
Boster Bio
Anti-LEF1 Antibody Picoband(r)A00605-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetLEF1
  • SizePrice
Anti-LEF1 Antibody
Product group Antibodies
Antibodies.com
Anti-LEF1 AntibodyA89721
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
LEF1 Antibody (YA3164, Rabbit)HY-P83419
TargetLEF1
  • SizePrice
Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.
Product group Antibodies
Atlas Antibodies
Anti-LEF1 AntibodyHPA002087
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetLEF1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
LEF1 AntibodyLS-C499233
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetLEF1
  • SizePrice
Product group Antibodies
Biomatik
Lef1 Polyclonal AntibodyCAC07199
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetLEF1
  • SizePrice
Mouse lymph node lysates probed with Rabbit Anti-LEF-1 Polyclonal Antibody, Unconjugated (bs-1843R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Product group Antibodies
Bioss
LEF-1 Polyclonal AntibodyBS-1843R
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
TargetLEF1
  • SizePrice
WB analysis of HeLa cells using GTX77781 LEF1 antibody at 0.2-1μg/ml.
Product group Antibodies
GeneTex
LEF1 antibody, N-termGTX77781
ApplicationsWestern Blot
ReactivityHuman
TargetLEF1
  • SizePrice