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Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using Galectin 8 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using Galectin 8 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using Galectin 8 Polyclonal Antibody at dilution 1:50

LGALS8 Polyclonal Antibody

E-AB-14094
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    LGALS8 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin 8 using anti-Galectin 8 antibody (PB9659). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates Lane 2: rat testicular tissue lysates Lane 3: mouse Neuro-2A whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 8 antigen affinity purified polyclonal antibody (Catalog # PB9659) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 8 at approximately 36KD. The expected band size for Galectin 8 is at 36KD.
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