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Western blot All lanes: LHX6 antibody at 3.02ug/ml Lane 1: HepG2 whole cell lysate Lane 2: U87 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Mouse brain tissue Lane 5: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 39, 44, 42, 20 kDa Observed band size: 41, 22, 20 kDa
Western blot All lanes: LHX6 antibody at 3.02ug/ml Lane 1: HepG2 whole cell lysate Lane 2: U87 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Mouse brain tissue Lane 5: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 39, 44, 42, 20 kDa Observed band size: 41, 22, 20 kDa
Western blot All lanes: LHX6 antibody at 3.02ug/ml Lane 1: HepG2 whole cell lysate Lane 2: U87 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Mouse brain tissue Lane 5: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 39, 44, 42, 20 kDa Observed band size: 41, 22, 20 kDa

LHX6 Antibody

CSB-PA891795ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetLHX6
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Overview

  • Supplier
    Cusabio
  • Product Name
    LHX6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26468
  • Target name
    LHX6
  • Target description
    LIM homeobox 6
  • Target synonyms
    LHX6.1; LIM homeodomain protein 6.1; LIM/homeobox protein Lhx6; LIM/homeobox protein Lhx6.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPM6
  • Protein Name
    LIM/homeobox protein Lhx6
  • Scientific Description
    Probable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex (By similarity).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LHX6 using anti-LHX6 antibody (A07568-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX6 antigen affinity purified polyclonal antibody (Catalog # A07568-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHX6 at approximately 60 kDa. The expected band size for LHX6 is at 40 kDa.
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