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LHX6 Polyclonal Antibody

BS-7869R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLHX6
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Overview

  • Supplier
    Bioss
  • Product Name
    LHX6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID26468
  • Target name
    LHX6
  • Target description
    LIM homeobox 6
  • Target synonyms
    LHX6.1, hLHX6, LIM/homeobox protein Lhx6, LIM homeodomain protein 6.1, LIM/homeobox protein Lhx6.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: LHX6 antibody at 3.02ug/ml Lane 1: HepG2 whole cell lysate Lane 2: U87 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Mouse brain tissue Lane 5: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 39, 44, 42, 20 kDa Observed band size: 41, 22, 20 kDa
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WB analysis of various sample lysates using GTX55690 LHX6 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
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Figure 1. Western blot analysis of LHX6 using anti-LHX6 antibody (A07568-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX6 antigen affinity purified polyclonal antibody (Catalog # A07568-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHX6 at approximately 60 kDa. The expected band size for LHX6 is at 40 kDa.
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