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Western Blot analysis of SKOV3 cells using LIR-1 Polyclonal Antibody
Western Blot analysis of SKOV3 cells using LIR-1 Polyclonal Antibody
Western Blot analysis of SKOV3 cells using LIR-1 Polyclonal Antibody

LILRB1 Antibody

CSB-PA006214
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetLILRB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LILRB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10859
  • Target name
    LILRB1
  • Target description
    leukocyte immunoglobulin like receptor B1
  • Target synonyms
    CD85J, ILT-2, ILT2, LIR-1, LIR1, MIR-7, MIR7, PIR-B, PIRB, leukocyte immunoglobulin-like receptor subfamily B member 1, CD85 antigen-like family member J, Ig-like transcript 2, immunoglobulin heavy chain variable region, leucocyte Ig-like receptor B1, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1, monocyte/macrophage immunoglobulin-like receptor 7, myeloid inhibitory receptor 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NHL6
  • Protein Name
    Leukocyte immunoglobulin-like receptor subfamily B member 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LILRB1 using anti-LILRB1 antibody (A03208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LILRB1 antigen affinity purified polyclonal antibody (Catalog # A03208-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LILRB1 at approximately 71 kDa. The expected band size for LILRB1 is at 71 kDa.
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