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ProductsBack/LIMK-2 (A-12) Alexa Fluor(r) 488

LIMK-2 (A-12) Alexa Fluor(r) 488

SC-365414AF488
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLIMK2
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    LIMK-2 (A-12) Alexa Fluor(r) 488
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 488
  • Gene ID3985
  • Target name
    LIMK2
  • Target description
    LIM domain kinase 2
  • Target synonyms
    LIM domain kinase 2
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of LIM kinase 2 using anti-LIM kinase 2 antibody (PB9717). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Brain Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: Mouse Thymus Tissue Lysate at 50ug, Lane 4: Mouse Testis Tissue Lysate at 50ug, Lane 5: 293T Whole Cell Lysate at 40ug, Lane 6: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIM kinase 2 antigen affinity purified polyclonal antibody (Catalog # PB9717) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIM kinase 2 at approximately 72 kDa. The expected band size for LIM kinase 2 is at 72 kDa.
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