Bio-Connect

LIN7C / VELI3 Antibody (N-Terminus)

LS-B3550
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLIN7C
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LIN7C / VELI3 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Antibody Specificity
    Human LIN7C
  • Application Supplier Note
    Immunohistochemistry: LS-B3550 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3550 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.. IHC, IHC-P (5 µg/ml), WB Immunohistochemistry: LS-B3550 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3550 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55327
  • Target name
    LIN7C
  • Target description
    lin-7 homolog C, crumbs cell polarity complex component
  • Target synonyms
    LIN-7 protein 3; LIN-7C; LIN-7-C; MALS3; MALS-3; mammalian lin-seven protein 3; protein lin-7 homolog C; VELI3; veli-3; vertebrate lin-7 homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203