LIN7C / VELI3 Antibody (N-Terminus)
LS-B3550
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLIN7C
Overview
- SupplierLifeSpan BioSciences
- Product NameLIN7C / VELI3 Antibody (N-Terminus)
- Delivery Days Customer14
- Antibody SpecificityHuman LIN7C
- Application Supplier NoteImmunohistochemistry: LS-B3550 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3550 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.. IHC, IHC-P (5 µg/ml), WB Immunohistochemistry: LS-B3550 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3550 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Estimated Purity...
- Gene ID55327
- Target nameLIN7C
- Target descriptionlin-7 homolog C, crumbs cell polarity complex component
- Target synonymsLIN-7 protein 3; LIN-7C; LIN-7-C; MALS3; MALS-3; mammalian lin-seven protein 3; protein lin-7 homolog C; VELI3; veli-3; vertebrate lin-7 homolog 3
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203