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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA032798(LLGL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA032798(LLGL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA032798(LLGL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

LLGL2 Antibody

CSB-PA032798
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetLLGL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LLGL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3993
  • Target name
    LLGL2
  • Target description
    LLGL scribble cell polarity complex component 2
  • Target synonyms
    HGL, Hugl-2, LGL2, LLGL scribble cell polarity complex component 2, LLGL2, scribble cell polarity complex component, human giant larvae homolog, lethal giant larvae homolog 2, scribble cell polarity complex component, lethal(2) giant larvae protein homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P1M3
  • Protein Name
    LLGL scribble cell polarity complex component 2
  • Scientific Description
    The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the proteins ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LLGL2 using anti-LLGL2 antibody (A08503-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LLGL2 antigen affinity purified polyclonal antibody (Catalog # A08503-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LLGL2 at approximately 120 kDa. The expected band size for LLGL2 is at 113 kDa.
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