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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA032798(LLGL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA032798(LLGL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA032798(LLGL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

LLGL2 Antibody

CSB-PA032798
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLLGL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LLGL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3993
  • Target name
    LLGL2
  • Target description
    LLGL scribble cell polarity complex component 2
  • Target synonyms
    HGL; Hugl-2; human giant larvae homolog; lethal giant larvae homolog 2, scribble cell polarity complex component; lethal(2) giant larvae protein homolog 2; LGL2; LLGL scribble cell polarity complex component 2; LLGL2, scribble cell polarity complex component
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P1M3
  • Protein Name
    Lethal(2) giant larvae protein homolog 2
  • Scientific Description
    The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the proteins ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LLGL2 using anti-LLGL2 antibody (A08503-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LLGL2 antigen affinity purified polyclonal antibody (Catalog # A08503-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LLGL2 at approximately 120 kDa. The expected band size for LLGL2 is at 113 kDa.
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