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LLGL2 Antibody

LS-C748377
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLLGL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LLGL2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 114kDa, while the observed MW by Western blot was 114kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 114kDa, while the observed MW by Western blot was 114kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3993
  • Target name
    LLGL2
  • Target description
    LLGL scribble cell polarity complex component 2
  • Target synonyms
    HGL, Hugl-2, LGL2, LLGL scribble cell polarity complex component 2, LLGL2, scribble cell polarity complex component, human giant larvae homolog, lethal giant larvae homolog 2, scribble cell polarity complex component, lethal(2) giant larvae protein homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LLGL2 using anti-LLGL2 antibody (A08503-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LLGL2 antigen affinity purified polyclonal antibody (Catalog # A08503-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LLGL2 at approximately 120 kDa. The expected band size for LLGL2 is at 113 kDa.
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