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Western Blot analysis of HepG2 cells using Lamin A/C Polyclonal Antibody
Western Blot analysis of HepG2 cells using Lamin A/C Polyclonal Antibody
Western Blot analysis of HepG2 cells using Lamin A/C Polyclonal Antibody

LMNA Antibody

CSB-PA003130
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLMNA
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Overview

  • Supplier
    Cusabio
  • Product Name
    LMNA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4000
  • Target name
    LMNA
  • Target description
    lamin A/C
  • Target synonyms
    CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1, lamin, 70 kDa lamin, epididymis secretory sperm binding protein, lamin A/C-like 1, lamin C, mandibuloacral dysplasia type A, prelamin-A/C, progerin, renal carcinoma antigen NY-REN-32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02545
  • Protein Name
    Prelamin-A/C
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified polyclonal antibody (Catalog # PB9280) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin A/C at approximately 74 kDa, 63 kDa. The expected band size for Lamin A/C is at 74 kDa.
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