Mouse anti Lamin A

Nordic-MUbio

Mouse anti Lamin A

7

133A2 recognizes an epitope located between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A.

133A2 is suitable for immunoblotting, immunocytochemistry on permeabilized cells, immunohistochemistry on frozen sections and paraffin embedded tissues, and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunocytochemistry, and immunohistochemistry with fluorescent secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

133A2

LMNA

lamin A/C

70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C-like 1; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; prelamin-A/C; PRO1; renal carcinoma antigen NY-REN-32

Mouse

IgG3

Prelamin-A/C

133A2 is a mouse monoclonal IgG3/kappa antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.

Bovine, Canine, Human, Mouse, Rat

Bovine;Canine;Human;Mouse;Rat

12352203