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Western blot All lanes: LMO2 antibody at 8 ug/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19, 26, 6 kDa Observed band size: 19 kDa
Western blot All lanes: LMO2 antibody at 8 ug/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19, 26, 6 kDa Observed band size: 19 kDa
Western blot All lanes: LMO2 antibody at 8 ug/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19, 26, 6 kDa Observed band size: 19 kDa

LMO2 Antibody

CSB-PA013009HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetLMO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LMO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4005
  • Target name
    LMO2
  • Target description
    LIM domain only 2
  • Target synonyms
    LMO-2, RBTN2, RBTNL1, RHOM2, TTG2, rhombotin-2, LIM domain only protein 2, T-cell translocation gene 2, T-cell translocation protein 2, cysteine-rich protein TTG-2, rhombotin-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25791
  • Protein Name
    Rhombotin-2
  • Scientific Description
    Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SMMC-7721 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.
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