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LMO2 (B-Cell Marker)(rLMO2/1971), CF594 conjugate, 0.1mg/mL [26628-22-8]

LMO2 (B-Cell Marker)(rLMO2/1971), CF594 conjugate, 0.1mg/mL [26628-22-8]

BNC943806
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLMO2
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Overview

  • Supplier
    Biotium
  • Product Name
    LMO2 (B-Cell Marker)(rLMO2/1971), CF594 conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    rLMO2/1971
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID4005
  • Target name
    LMO2
  • Target description
    LIM domain only 2
  • Target synonyms
    cysteine-rich protein TTG-2; LIM domain only protein 2; LMO-2; RBTN2; RBTNL1; RHOM2; rhombotin-2; rhombotin-like 1; T-cell translocation gene 2; T-cell translocation protein 2; TTG2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP25791
  • Protein Name
    Rhombotin-2
  • Scientific Description
    The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. It has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymph-angiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma. The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin. LMO2 is useful in differentiating follicular lymphoma (LMO2 ) from nodal marginal zone lymphoma (LM02-). It also is positive in Hodgkins and Burkitts lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SMMC-7721 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.
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