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Western Blot analysis of 3T3 cells using LMTK3 Polyclonal Antibody
Western Blot analysis of 3T3 cells using LMTK3 Polyclonal Antibody
Western Blot analysis of 3T3 cells using LMTK3 Polyclonal Antibody

LMTK3 Antibody

CSB-PA006834
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Cusabio
  • Product Name
    LMTK3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96Q04
  • Protein Name
    Serine/threonine-protein kinase LMTK3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LMTK3 using anti-LMTK3 antibody (A08432-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human PC-3 whole cell lysates Lane 3: human U2OS whole cell lysates Lane 4: mouse thymus tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMTK3 antigen affinity purified polyclonal antibody (Catalog # A08432-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMTK3 at approximately 153KD. The expected band size for LMTK3 is at 153KD.
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