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Loricrin antibody [N1], N-term

GTX116013
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLORICRIN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Loricrin antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.24 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4014
  • Target name
    LORICRIN
  • Target description
    loricrin cornified envelope precursor protein
  • Target synonyms
    LOR; loricrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23490
  • Protein Name
    Loricrin
  • Scientific Description
    This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkels syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice. Oh JS et al., 2021 Sep 15, Nutrients
    Read more
  • Contribution of transglutaminases and their substrate proteins to the formation of cornified cell envelope in oral mucosal epithelium. Roy RR et al., 2021 Feb, Eur J Oral Sci
    Read more
  • Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function. Kim SH et al., 2020 Jan 18, Nutrients
    Read more
  • MiR-744-3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis. Wang C et al., 2019 Mar, Exp Dermatol
    Read more
  • Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes. Wang S et al., 2018 Oct, Med Sci (Paris)
    Read more
  • Ectopic transglutaminase 1 and 3 expression accelerating keratinization in oral lichen planus. Shimada K et al., 2018 Nov, J Int Med Res
    Read more
  • LncRNA expression profile of deltaNp63alpha in cervical squamous cancers and its suppressive effects on LIF expression. Qian L et al., 2017 Aug, Cytokine
    Read more
  • Downregulation of miR-205 in migrating epithelial tongue facilitates skin wound re-epithelialization by derepressing ITGA5. Wang T et al., 2016 Aug, Biochim Biophys Acta
    Read more
  • Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response. Dang NN et al., 2015 Jan, Braz J Med Biol Res
    Read more

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Figure 1. Western blot analysis of LOR using anti-LOR antibody (A05290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human U-937 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat heart tissue lysates Lane 6: rat small intestine tissue lysates Lane 7: mouse heart tissue lysates Lane 8: mouse small intestine tissue lysates Lane 9: mouse Neuro-2a whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOR antigen affinity purified polyclonal antibody (Catalog # A05290-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOR at approximately 26KD. The expected band size for LOR is at 26KD.
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