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ProductsBack/LOR/Loricrin Recombinant Antibody, AbBy Fluor-350 Conjugated

LOR/Loricrin Recombinant Antibody, AbBy Fluor-350 Conjugated

BSM-61744R-BF350
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetLORICRIN
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    LOR/Loricrin Recombinant Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID4014
  • Target name
    LORICRIN
  • Target description
    loricrin cornified envelope precursor protein
  • Target synonyms
    LOR; loricrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23490
  • Protein Name
    Loricrin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LOR using anti-LOR antibody (A05290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human U-937 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat heart tissue lysates Lane 6: rat small intestine tissue lysates Lane 7: mouse heart tissue lysates Lane 8: mouse small intestine tissue lysates Lane 9: mouse Neuro-2a whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOR antigen affinity purified polyclonal antibody (Catalog # A05290-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOR at approximately 26KD. The expected band size for LOR is at 26KD.
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