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Immunofluorescent image of a cross-section of a zebrafish embryo using LOXL1 antibody (GTX114532) at a 1:200 dilution. LOXL1(Blue) a-Tubulin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Immunofluorescent image of a cross-section of a zebrafish embryo using LOXL1 antibody (GTX114532) at a 1:200 dilution. LOXL1(Blue) a-Tubulin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Immunofluorescent image of a cross-section of a zebrafish embryo using LOXL1 antibody (GTX114532) at a 1:200 dilution. LOXL1(Blue) a-Tubulin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

LOXL1 antibody

GTX114532
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetLOXL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    LOXL1 antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4016
  • Target name
    LOXL1
  • Target description
    lysyl oxidase like 1
  • Target synonyms
    LOL, LOXL, lysyl oxidase homolog 1, lysyl oxidase-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08397
  • Protein Name
    Lysyl oxidase homolog 1
  • Scientific Description
    This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]
  • Reactivity
    Human, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Xu C, Chi Q, Yang L, et al. Effect of mechanical injury and IL-1β on the expression of LOXs and MMP-1, 2, 3 in PCL fibroblasts after co-culture with synoviocytes. Gene. 2021,766:145149. doi: 10.1016/j.gene.2020.145149
    Read this paper
  • Wang C, Chi Q, Sha Y, et al. Mechanical injury and IL-1β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes. Biotechnol Lett. 2020,42(8):1567-1579. doi: 10.1007/s10529-020-02870-9
    Read this paper
  • Wang C, Xu C, Chen R, et al. Different expression profiles of the lysyl oxidases and matrix metalloproteinases in human ACL fibroblasts after co-culture with synovial cells. Connect Tissue Res. 2018,59(4):369-380. doi: 10.1080/03008207.2017.1401615
    Read this paper
  • Jung IH, Park JC, Kim JC, et al. Novel application of human periodontal ligament stem cells and water-soluble chitin for collagen tissue regeneration: in vitro and in vivo investigations. Tissue Eng Part A. 2012,18(5-6):643-53. doi: 10.1089/ten.TEA.2011.0164
    Read this paper

Datasheet

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Figure 1. Western blot analysis of LOXL1 using anti-LOXL1 antibody (PB9719). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL1 antigen affinity purified polyclonal antibody (Catalog # PB9719) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL1 at approximately 63 kDa. The expected band size for LOXL1 is at 63 kDa.
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