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Western Blot analysis of 3T3 cells using EDG-7 Polyclonal Antibody
Western Blot analysis of 3T3 cells using EDG-7 Polyclonal Antibody
Western Blot analysis of 3T3 cells using EDG-7 Polyclonal Antibody

LPAR3 Antibody

CSB-PA002255
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLPAR3
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Overview

  • Supplier
    Cusabio
  • Product Name
    LPAR3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23566
  • Target name
    LPAR3
  • Target description
    lysophosphatidic acid receptor 3
  • Target synonyms
    calcium-mobilizing lysophosphatidic acid receptor LP-A3; EDG7; Edg-7; endothelial cell differentiation gene 7; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7; GPCR; HOFNH30; LPA receptor 3; LPA receptor EDG7; LPA3; LP-A3; LPA-3; lysophosphatidic acid receptor 3; lysophosphatidic acid receptor Edg-7; RP4-678I3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBY5
  • Protein Name
    Lysophosphatidic acid receptor 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EDG7/LPAR3 using anti-EDG7/LPAR3 antibody (A06597-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG7/LPAR3 antigen affinity purified polyclonal antibody (Catalog # A06597-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG7/LPAR3 at approximately 43 kDa. The expected band size for EDG7/LPAR3 is at 40 kDa.
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