More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/LPAR3 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

LPAR3 Polyclonal Antibody

BS-2882R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLPAR3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    LPA3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23566
  • Target name
    LPAR3
  • Target description
    lysophosphatidic acid receptor 3
  • Target synonyms
    calcium-mobilizing lysophosphatidic acid receptor LP-A3; EDG7; Edg-7; endothelial cell differentiation gene 7; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7; GPCR; HOFNH30; LPA receptor 3; LPA receptor EDG7; LPA3; LP-A3; LPA-3; lysophosphatidic acid receptor 3; lysophosphatidic acid receptor Edg-7; RP4-678I3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBY5
  • Protein Name
    Lysophosphatidic acid receptor 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Lysophosphatidic acid decreased macrophage foam cell migration correlated with downregulation of fucosyltransferase 8 via HNF1alpha. Chen L et al., 2019 Nov, Atherosclerosis
    Read more
  • Lysophosphatidic acid directly induces macrophage-derived foam cell formation by blocking the expression of SRBI. Chen L et al., 2017 Sep 23, Biochem Biophys Res Commun
    Read more

Related products

Anti-EDG7 Antibody
Product group Antibodies
Antibodies.com
Anti-EDG7 AntibodyA100003
ApplicationsImmunoFluorescence, Western Blot, ELISA
  • SizePrice
Western Blot analysis of 3T3 cells using EDG-7 Polyclonal Antibody
Product group Antibodies
Cusabio
LPAR3 AntibodyCSB-PA002255
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetLPAR3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
LPAR3 / LPA3 / EDG7 AntibodyLS-C406081
ApplicationsELISA, ImmunoHistoChemistry
TargetLPAR3
  • SizePrice
Figure 1. Western blot analysis of EDG7/LPAR3 using anti-EDG7/LPAR3 antibody (A06597-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG7/LPAR3 antigen affinity purified polyclonal antibody (Catalog # A06597-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG7/LPAR3 at approximately 43 kDa. The expected band size for EDG7/LPAR3 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-LPAR3 Antibody Picoband(r)A06597-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetLPAR3
  • SizePrice
Product group Antibodies
Nordic-MUbio
Rabbit anti Human Lysophosphatidic Acid Receptor 3 CT (EDG-7)X1183P
ApplicationsWestern Blot
TargetLPAR3
  • SizePrice