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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA3) Polyclonal Antibody, Unconjugated (bs-2882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

LPAR3 Polyclonal Antibody

BS-2882R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLPAR3
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Overview

  • Supplier
    Bioss
  • Product Name
    LPAR3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23566
  • Target name
    LPAR3
  • Target description
    lysophosphatidic acid receptor 3
  • Target synonyms
    EDG7, Edg-7, GPCR, HOFNH30, LP-A3, LPA3, RP4-678I3, lysophosphatidic acid receptor 3, LPA receptor 3, LPA receptor EDG7, LPA-3, calcium-mobilizing lysophosphatidic acid receptor LP-A3, endothelial cell differentiation gene 7, endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7, lysophosphatidic acid receptor Edg-7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBY5
  • Protein Name
    Lysophosphatidic acid receptor 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chen L, Zhang J, Yang X, et al. Lysophosphatidic acid decreased macrophage foam cell migration correlated with downregulation of fucosyltransferase 8 via HNF1α. Atherosclerosis. 2019,290:19-30. doi: 10.1016/j.atherosclerosis.2019.09.001
    Read this paper
  • Chen L, Zhang J, Deng X, et al. Lysophosphatidic acid directly induces macrophage-derived foam cell formation by blocking the expression of SRBI. Biochem Biophys Res Commun. 2017,491(3):587-594. doi: 10.1016/j.bbrc.2017.07.159
    Read this paper

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Figure 1. Western blot analysis of EDG7/LPAR3 using anti-EDG7/LPAR3 antibody (A06597-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG7/LPAR3 antigen affinity purified polyclonal antibody (Catalog # A06597-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG7/LPAR3 at approximately 43 kDa. The expected band size for EDG7/LPAR3 is at 40 kDa.
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