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Western Blot analysis of K562 cells using GPR92 Polyclonal Antibody
Western Blot analysis of K562 cells using GPR92 Polyclonal Antibody
Western Blot analysis of K562 cells using GPR92 Polyclonal Antibody

LPAR5 Antibody

CSB-PA002795
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLPAR5
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Overview

  • Supplier
    Cusabio
  • Product Name
    LPAR5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57121
  • Target name
    LPAR5
  • Target description
    lysophosphatidic acid receptor 5
  • Target synonyms
    G protein-coupled receptor 92; GPR92; GPR93; G-protein coupled receptor 93; KPG_010; LPA receptor 5; LPA5; LPA-5; lysophosphatidic acid receptor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1C0
  • Protein Name
    Lysophosphatidic acid receptor 5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GPR92/LPAR5 using anti-GPR92/LPAR5 antibody (A06721-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR92/LPAR5 antigen affinity purified polyclonal antibody (Catalog # A06721-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR92/LPAR5 at approximately 41 kDa. The expected band size for GPR92/LPAR5 is at 41 kDa.
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