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ProductsBack/LPAR5 / GPR92 Antibody (210-290 aa, Internal)

LPAR5 / GPR92 Antibody (210-290 aa, Internal)

LS-C383820
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLPAR5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LPAR5 / GPR92 Antibody (210-290 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000), IF (1:200 - 1:1000), IHC (1:100 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57121
  • Target name
    LPAR5
  • Target description
    lysophosphatidic acid receptor 5
  • Target synonyms
    GPR92, GPR93, KPG_010, LPA5, lysophosphatidic acid receptor 5, G protein-coupled receptor 92, G-protein coupled receptor 93, LPA receptor 5, LPA-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GPR92/LPAR5 using anti-GPR92/LPAR5 antibody (A06721-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR92/LPAR5 antigen affinity purified polyclonal antibody (Catalog # A06721-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR92/LPAR5 at approximately 41 kDa. The expected band size for GPR92/LPAR5 is at 41 kDa.
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