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ProductsBack/LSD2 / AOF1 antibody [HL3728]
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LSD2 / AOF1 antibody [HL3728] (GTX641918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LSD2 / AOF1 antibody [HL3728] (GTX641918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LSD2 / AOF1 antibody [HL3728] (GTX641918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LSD2 / AOF1 antibody [HL3728]

GTX641918
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetKDM1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    LSD2 / AOF1 antibody [HL3728]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL3728
  • Conjugate
    Unconjugated
  • Gene ID221656
  • Target name
    KDM1B
  • Target description
    lysine demethylase 1B
  • Target synonyms
    AOF1, C6orf193, LSD2, lysine-specific histone demethylase 2, flavin-containing amine oxidase domain-containing protein 1, lysine (K)-specific demethylase 1B, lysine-specific histone demethylase 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB78
  • Protein Name
    Lysine-specific histone demethylase 2
  • Scientific Description
    Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KDM1B using anti-KDM1B antibody (M08071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM1B antigen affinity purified monoclonal antibody (Catalog # M08071) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM1B at approximately 93 kDa. The expected band size for KDM1B is at 93 kDa.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LSD2 / AOF1 antibody [HL3729] (GTX641919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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