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ProductsBack/Ly-6A/E antibody [D7]

Ly-6A/E antibody [D7]

GTX30898
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetLy6a
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ly-6A/E antibody [D7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    For FACS: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D7
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID110454
  • Target name
    Ly6a
  • Target description
    lymphocyte antigen 6 family member A
  • Target synonyms
    Ly-6A.2, Ly-6A/E, Ly-6E.1, Sca-1, Sca1, TAP, lymphocyte antigen 6A-2/6E-1, T-cell-activating protein, lymphocyte antigen 6 complex, locus A, stem cell antigen 1
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP05533
  • Protein Name
    Lymphocyte antigen 6A-2/6E-1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • English A, Kosoy R, Pawlinski R, et al. A monoclonal antibody against the 66-kDa protein expressed in mouse spleen and thymus inhibits Ly-6A.2-dependent cell-cell adhesion. J Immunol. 2000,165(7):3763-71.
    Read this paper

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Figure 1. Western blot analysis of Sca1/Ly6A/E using anti-Sca1/Ly6A/E antibody (A30403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sca1/Ly6A/E antigen affinity purified polyclonal antibody (Catalog # A30403) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sca1/Ly6A/E at approximately 14KD. The expected band size for Sca1/Ly6A/E is at 14KD.
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