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Ly6c1 Antibody (clone Monts 1, Azide-free, Low Endotoxin)

LS-C812187
LifeSpan BioSciences
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
TargetLy6c1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Ly6c1 Antibody (clone Monts 1, Azide-free, Low Endotoxin)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 microg per 106 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Depl, Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Monts 1
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID17067
  • Target name
    Ly6c1
  • Target description
    lymphocyte antigen 6 complex, locus C1
  • Target synonyms
    AA682074; AA959465; Ly6; Ly-6; Ly6c; Ly-6C; Ly-6C1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LY6C using anti-LY6C antibody (A33313). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6C antigen affinity purified polyclonal antibody (Catalog # A33313) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6C at approximately 14KD. The expected band size for LY6C is at 14KD.
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