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LY6E antibody

ORB374543
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetLY6E
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Overview

  • Supplier
    Biorbyt
  • Product Name
    LY6E antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    WB: 1:1000-2000 ELISA, IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4061
  • Target name
    LY6E
  • Target description
    lymphocyte antigen 6 family member E
  • Target synonyms
    RIG-E, RIGE, SCA-2, SCA2, TSA-1, lymphocyte antigen 6E, ly-6E, lymphocyte antigen 6 complex, locus E, retinoic acid induced gene E, retinoic acid-induced gene E protein, stem cell antigen 2, thymic shared antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16553
  • Protein Name
    Lymphocyte antigen 6E
  • Scientific Description
    Rabbit polyclonal antibody to LY6E
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Non-transfected (–) and transfected (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of LY6E/SCA-2 using anti-LY6E/SCA-2 antibody (A09496-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6E/SCA-2 antigen affinity purified polyclonal antibody (Catalog # A09496-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6E/SCA-2 at approximately 14 kDa. The expected band size for LY6E/SCA-2 is at 14 kDa.
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