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Western Blot analysis of HeLa cells using Lyn Polyclonal Antibody
Western Blot analysis of HeLa cells using Lyn Polyclonal Antibody
Western Blot analysis of HeLa cells using Lyn Polyclonal Antibody

LYN Antibody

CSB-PA003180
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLYN
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Overview

  • Supplier
    Cusabio
  • Product Name
    LYN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4067
  • Target name
    LYN
  • Target description
    LYN proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    JTK8, SAIDV, p53Lyn, p56Lyn, tyrosine-protein kinase Lyn, lck/Yes-related novel protein tyrosine kinase, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07948
  • Protein Name
    Tyrosine-protein kinase Lyn
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 5. IHC analysis of Lyn using anti-Lyn antibody (A01424-2). Lyn was detected in paraffin-embedded section of mouse intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Lyn Antibody (A01424-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Lane 1: Mouse SP2/0 cell lysates probed with Lyn Polyclonal Antibody, Unconjugated (bs-2906R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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