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LYN antibody detects LYN protein by Western blot analysis. A. 30 μg Raji whole cell lysate/extract B. 30 μg K562 whole cell lysate/extract C. 30 μg Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody (GTX101222) dilution: 1:1000
LYN antibody detects LYN protein by Western blot analysis. A. 30 μg Raji whole cell lysate/extract B. 30 μg K562 whole cell lysate/extract C. 30 μg Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody (GTX101222) dilution: 1:1000
LYN antibody detects LYN protein by Western blot analysis. A. 30 μg Raji whole cell lysate/extract B. 30 μg K562 whole cell lysate/extract C. 30 μg Raji whole cell lysate/extract 7.5 % SDS-PAGE LYN antibody (GTX101222) dilution: 1:1000

LYN antibody

GTX101222
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetLYN
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Overview

  • Supplier
    GeneTex
  • Product Name
    LYN antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4067
  • Target name
    LYN
  • Target description
    LYN proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    JTK8, SAIDV, p53Lyn, p56Lyn, tyrosine-protein kinase Lyn, lck/Yes-related novel protein tyrosine kinase, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07948
  • Protein Name
    Tyrosine-protein kinase Lyn
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ben-Khemis M, Liu D, Pintard C, et al. TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes. Redox Biol. 2023,67:102898. doi: 10.1016/j.redox.2023.102898
    Read this paper
  • Li JP, Yang CY, Chuang HC, et al. The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck. Nat Commun. 2014,5:3618. doi: 10.1038/ncomms4618
    Read this paper
  • Lai WB, Wang BJ, Hu MK, et al. Ligand-dependent activation of EphA4 signaling regulates the proteolysis of amyloid precursor protein through a Lyn-mediated pathway. Mol Neurobiol. 2014,49(2):1055-68. doi: 10.1007/s12035-013-8580-x
    Read this paper

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Figure 5. IHC analysis of Lyn using anti-Lyn antibody (A01424-2). Lyn was detected in paraffin-embedded section of mouse intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Lyn Antibody (A01424-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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