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Western Blot analysis of COLO205 cells using LYPLA1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using LYPLA1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using LYPLA1 Polyclonal Antibody

LYPLA1 Antibody

CSB-PA003181
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLYPLA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LYPLA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10434
  • Target name
    LYPLA1
  • Target description
    lysophospholipase 1
  • Target synonyms
    APT-1, APT1, LPL-I, LPL1, hAPT1, acyl-protein thioesterase 1, lysoPLA I, lysophospholipase I, lysophospholipid-specific lysophospholipase, palmitoyl-protein hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75608
  • Protein Name
    Acyl-protein thioesterase 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LYPLA1 using anti-LYPLA1 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA1 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA1 at approximately 25 kDa. The expected band size for LYPLA1 is at 25 kDa.
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