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Lysophospholipase 1 Antibody (YA2197, Rabbit)

HY-P82452
MedChem Express
TargetLYPLA1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Lysophospholipase 1 Antibody (YA2197, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10434
  • Target name
    LYPLA1
  • Target description
    lysophospholipase 1
  • Target synonyms
    acyl-protein thioesterase 1; APT1; APT-1; hAPT1; LPL1; LPL-I; lysophospholipase I; lysophospholipid-specific lysophospholipase; lysoPLA I
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Lysophospholipase 1 Antibody (YA2197) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2197), targeting Lysophospholipase 1, with a predicted molecular weight of 25 kDa (observed band size: 25 kDa). Lysophospholipase 1 Antibody (YA2197) can be used for WB, ICC/IF, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LYPLA1 using anti-LYPLA1 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA1 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA1 at approximately 25 kDa. The expected band size for LYPLA1 is at 25 kDa.
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