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Immunofluorescent analysis of Hela cells using CSB-PA05074A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using CSB-PA05074A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using CSB-PA05074A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

LYPLA2 Antibody

CSB-PA05074A0RB
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetLYPLA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LYPLA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11313
  • Target name
    LYPLA2
  • Target description
    lysophospholipase 2
  • Target synonyms
    APT-2, APT2, DJ886K2.4, acyl-protein thioesterase 2, LPL-II, lysoPLA II, lysophospholipase II, palmitoyl-protein hydrolase, testicular tissue protein Li 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95372
  • Protein Name
    Acyl-protein thioesterase 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LYPLA2 antibody [N1C3] (GTX102240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of LYPLA2 using anti-LYPLA2 antibody (A10621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA2 antigen affinity purified polyclonal antibody (Catalog # A10621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA2 at approximately 25 kDa. The expected band size for LYPLA2 is at 25 kDa.
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